“Bunyaviral translation strategies”,
Laboratory of Structural Virology
Head: Piotr Gerlach
POSITION IN PROJECT
Structural biology, molecular biology, biochemistry.
NUMBER OF POSITIONS
“Regenerative Mechanisms for Health" ReMedy
“Bunyaviral translation strategies”
Head: Piotr Gerlach / Laboratory of Structural Virology
Host translation is one of the major sites of the virus-host battlefront and a center of cellular stress response pathways triggered during viral infection. The aim of this project is to investigate how segmented negative-strand RNA bunyaviruses manipulate and reprogramme cellular host translation during infection.
Methodologically we plan to integrate structural biology techniques (cryo-EM, cryo-ET, and X-ray crystallography) with biochemistry and in vivo approaches. Our lab has access to state-of-the-art instrumentation, including cell culture facilities, recombinant protein purification systems, mass spectrometry, and a Glacios cryo-TEM equipped with Falcon 3EC camera. Collaborations within the Ochota Life Science campus afford access to two FIB-SEM instruments for cryo-ET sample preparation. Finally, we will have access to a Titan Krios G3i cryo-TEM equipped with both K3 and Falcon3EC cameras, located at the National Centre for Electron Cryomicroscopy in Kraków.
The successful candidate will be involved in setting up an in vivo platform – mammalian cell cultures transfected with viral mini-replicon systems, which will be exploited in functional and structural characterization of the virus-host intracellular interactions. Experience in mammalian cell cultures, including purification of native protein complexes, is desired. Experience in cryo-EM/ET sample preparation and data processing would be an advantage.
Full-time position, stipend/contract
8 900,00 – 11 650,00 PLN gross, depending on experience
POSITION STARTS ON
1st Jul 2021
25th Apr 2021
YOUR FUTURE KEY RESPONSIBILITIES:
Perform experiments using biochemical, structural and molecular biology techniques (independently and in collaboration with other team members),
Participate in experimental design and data analysis,
Participate in the data preparation and writing of manuscripts,
Actively participate in lab meetings, scientific seminars and conferences.
IF YOU ARE:
PhD degree in molecular biology, biophysics, biochemistry or related fields of life sciences,
Solid knowledge and familiarity with laboratory techniques in at least one of the following disciplines: molecular biology, biochemistry, cell biology, genetics, transcriptomics,
Very good command of English,
Motivation and passion for experimental work.
... PLEASE APPLY.
And let us present the details to you.
Work in a young, active, team in an excellent scientific environment,
Comprehensive training in genetics, biochemistry and molecular biology,
Access to the state-of-the-art equipment and facilities,
Participation in courses and conferences.
REQUIRED DOCUMENTS (ENGLISH ONLY)
CV with the list of publications,
Contact details of three scientific supervisors or another referee/s
(including the direct PhD supervisor).
Please apply using the form below.
Please send all the documents as a single .pdf file.
We will contact selected candidates only.
Project is carried out within the International Research Agendas Programme of the Foundation for Polish Science
"Bunyaviral translation strategies"